Chemiluminescence biosensor for hydrogen peroxide determination by immobilizing horseradish peroxidase onto PVA-co-PE nanofiber membrane

Poly(ethylene-co-polyvinyl alcohol) (PVA-co-PE) nanofiber membrane with abundant active hydroxyl groups on the surface was modified through a solid-phase synthesis process. The PVA-co-PE nanofibers with different average diameters ranging from 200 to 500 nm were modified by cyanuric chloride and 1, 3-propanediamine. The aminated PVA-co-PE nanofiber membrane was subsequently biotinylated and then horseradish peroxidase (HRP) was successfully immobilized onto it. The morphologies, hydrophilicity and chemical structures of membranes surface were investigated by SEM, water contact angle, FTIR and XPS. The HRP-immobilized PVA-co-PE nanofiber membrane revealed high activity, efficiency, sensitivity and reusability when it was used to catalyze the luminol-H2O2 reaction. The results demonstrated that the HRP-immobilized PVA-co-PE nanofiber membrane could be a promising candidate as solid support materials for catalysis of Luminol-H2O2. The relative light unit (RLU) could run up to 5 x 10(5) when the H2O2 concentration was low to 1 x 10(-15) mol/L. The HRP-immobilized PVA-co-PE nanofiber membrane was the potential chemiluminescence biosensor which could be applied in biological, medical and food fields.