期刊
RSC ADVANCES
卷 11, 期 40, 页码 24843-24851出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d1ra04128b
关键词
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资金
- Undergraduate Innovation and Entrepreneurship Training Program [S202010650067]
- Comprehensive Health Management Promotion Center of Xihua University [DJKG2019-007]
A temperature-sensitive magnetic composite with good affinity to papain was successfully prepared, showing high adsorption capacity and recyclability. The composite could thermally protect papain and maintain its activity during adsorption and elution processes.
Herein, a novel temperature-sensitive magnetic composite (Fe3O4@SiO2@P(NIPAM-co-VI)/Cu2+) with a uniform core-shell-shell structure was successfully prepared via a layer-by-layer method. The resulting magnetic composite revealed good magnetic properties and remarkable affinity to papain with a maximum adsorption capacity of 199.17 mg g(-1). The adsorption equilibrium data fitted the pseudo-second-order kinetic and Freundlich models well, and the major thermodynamics parameters indicated that adsorption was an endothermic and spontaneous process. Fe3O4@SiO2@P(NIPAM-co-VI)/Cu2+ could thermally protect papain, which is attributed to the reversible hydrophilic-hydrophobic transition of the composite at temperatures below and above the lower critical solution temperature. More importantly, the magnetic composite could be recycled at least six times without a remarkable loss in its adsorption capacity, and the process of adsorption and elution had no significant effect on the activity and structure of papain. This work could provide a novel separation method for papain without loss of its activity.
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