Indolylbenzothiadiazoles as highly tunable fluorophores for imaging lipid droplet accumulation in astrocytes and glioblastoma cells

We present an extensive photophysical study of a series of fluorescent indolylbenzothiadiazole derivatives and their ability to specifically image lipid droplets in astrocytes and glioblastoma cells. All compounds in the series displayed positive solvatochromism together with large Stokes shifts, and pi-extended derivatives exhibited elevated brightness. It was shown that the fluorescence properties were highly tunable by varying the electronic character or size of the N-substituent on the indole motif. Three compounds proved capable as probes for detecting small quantities of lipid deposits in healthy and cancerous brain cells. In addition, all twelve compounds in the series were predicted to cross the blood-brain barrier, which raises the prospect for future in vivo studies for exploring the role of lipid droplets in the central nervous system.

Automated summary

This study conducted an extensive photophysical investigation of fluorescent derivatives and their ability to specifically image lipid droplets, proving them to be effective probes for detecting lipid deposits in both healthy and cancerous brain cells. Furthermore, all compounds in the series were predicted to cross the blood-brain barrier, opening up possibilities for future in vivo studies exploring the role of lipid droplets in the central nervous system.