4.1 Article

Directed movement toward, translocation along, penetration into and exit from vascular networks by breast cancer cells in 3D

期刊

CELL ADHESION & MIGRATION
卷 15, 期 1, 页码 224-248

出版社

TAYLOR & FRANCIS INC
DOI: 10.1080/19336918.2021.1957527

关键词

Filopodia; pseudopod; intravasation; extravasation; computer-assisted reconstruction; CD44; RHAMM

资金

  1. National Cancer Institute [P30CA086062]
  2. Developmental Studies Hybridoma Bank

向作者/读者索取更多资源

A computer-assisted platform was developed using laser scanning confocal microscopy to reconstruct interactions between metastatic breast cancer cells and human umbilical vein endothelial cells in real-time. Results showed that an anti-RHAMM antibody blocked filopodium formation and related behaviors.
We developed a computer-assisted platform using laser scanning confocal microscopy to 3D reconstruct in real-time interactions between metastatic breast cancer cells and human umbilical vein endothelial cells (HUVECs). We demonstrate that MB-231 cancer cells migrate toward HUVEC networks, facilitated by filopodia, migrate along the network surfaces, penetrate into and migrate within the HUVEC networks, exit and continue migrating along network surfaces. The system is highly amenable to 3D reconstruction and computational analyses, and assessments of the effects of potential anti-metastasis monoclonal antibodies and other drugs. We demonstrate that an anti-RHAMM antibody blocks filopodium formation and all of the behaviors that we found take place between MB-231 cells and HUVEC networks.

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