4.1 Article

Lepidium sativum as candidate against excitotoxicity in retinal ganglion cells

期刊

TRANSLATIONAL NEUROSCIENCE
卷 12, 期 1, 页码 247-259

出版社

DE GRUYTER POLAND SP Z O O
DOI: 10.1515/tnsci-2020-0174

关键词

Lepidium sativum; glutamate excitotoxicity; retinal ganglion cell; cell viability; COMET assay

资金

  1. Deanship of Scientific Research at King Saud University [RG-1441-540]

向作者/读者索取更多资源

The study found that L. sativum seed extracts can improve cell viability in retinal ganglion cells under the excitotoxic effects of glutamate and act as an anti-excitotoxic and antioxidant agent that may improve the clinical presentation of various neurological disorders.
Glutamate excitotoxicity is considered one of the major causes of retinal ganglion cell death in many retinal diseases. Retinal ganglion cell degeneration causes severe blindness since visual signals from the eye to the brain are conducted only through retinal ganglion cells. Objective: We aimed to explore the potential ameliorative effects of L. sativum against glutamate excitotoxicityinduced retinal ganglion cell damage. Methods: Pure retinal ganglion cells were divided into a control group (untreated); L. sativum-treated groups in which retinal ganglion cells were treated with 5, 10, 50, or 100 mu g/mL L. sativum seed extract for 2 h; glutamate-treated groups in which cells were treated with 5, 10, 50, or 100 mu M glutamate for 48 h; and L. sativum/glutamate groups [pretreatment with L. sativum for 2 h (50 or 100 mu g/mL) before glutamate treatment at 100 mu M for 48 h]. Cell damage was assessed by comet assay and cell viability was by MTT test. Results: Tailed DNA, tail length, and tail moment of the 50 and 100 mM glutamate-treated groups were significantly greater than those of the blank control group, while the L. sativum-treated groups demonstrated nonsignificantly different tailed DNA, tail length, and tail moment compared with the blank control group, but significantly lower values compared with the glutamate-treated groups. Conclusion: L. sativum ameliorated the cell viability in retinal ganglion cells after high-concentration glutamate exposure. L. sativum seed extracts were efficient anti-excitotoxic and antioxidant agent that might improve the clinical presentation of many neurological disorders.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.1
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据