期刊
CLINICAL & TRANSLATIONAL IMMUNOLOGY
卷 10, 期 7, 页码 -出版社
WILEY
DOI: 10.1002/cti2.1306
关键词
antibodies; antibody-secreting cells; circulating T follicular helper cells; COVID-19; germinal centres; SARS-CoV-2
类别
资金
- Marianne and Marcus Wallenberg Foundation
- Region Stockholm (ALF project)
- Region Stockholm
- Center for Innovative Medicine
- Karolinska Institutet
- Knut and Alice Wallenberg Foundation
- Nordstjernan AB
- Swedish Research Council
- Karolinska Institutet Foundations
The study observed an increase in germinal centre activity, substantial expansion of antibody-secreting cells, and generation of neutralizing antibodies during acute COVID-19. Despite decreasing antibody levels, persistent neutralizing antibody titres and specific memory B cell responses along with polyfunctional T cell responses were still observed at 5 and 9 months after symptom onset in both moderate and severe COVID-19 patients.
Objectives. Humoral and cellular immunity to SARS-CoV-2 following COVID-19 will likely contribute to protection from reinfection or severe disease. It is therefore important to characterise the initiation and persistence of adaptive immunity to SARS-CoV-2 amidst the ongoing pandemic. Methods. Here, we conducted a longitudinal study on hospitalised moderate and severe COVID-19 patients from the acute phase of disease into convalescence at 5 and 9 months post-symptom onset. Utilising flow cytometry, serological assays as well as B cell and T cell FluoroSpot assays, we assessed the magnitude and specificity of humoral and cellular immune responses during and after human SARS-CoV-2 infection. Results. During acute COVID-19, we observed an increase in germinal centre activity, a substantial expansion of antibody-secreting cells and the generation of SARS-CoV-2-neutralising antibodies. Despite gradually decreasing antibody levels, we show persistent, neutralising antibody titres as well as robust specific memory B cell responses and polyfunctional T cell responses at 5 and 9 months after symptom onset in both moderate and severe COVID-19 patients. Conclusion. Our findings describe the initiation and, importantly, persistence of cellular and humoral SARS-CoV-2-specific immunological memory in hospitalised COVID-19 patients long after recovery, likely contributing towards protection against reinfection.
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