期刊
EUROPEAN JOURNAL OF SOIL BIOLOGY
卷 81, 期 -, 页码 94-99出版社
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.ejsobi.2017.06.012
关键词
Carbon monoxide dehydrogenase; coxL gene; Abundance; Phylogeny; Soils
资金
- National Natural Science Foundation of China [41503081, 41522107]
- Australia-China Joint Research Centre-Healthy Soils for Sustainable Food Production and Environmental Quality [ACSRF48165]
- Recruitment Program of High-end Foreign Experts of the State Administration of Foreign Experts Affairs [GDT20164300013]
- Youth Innovation Team Project of Institute of Subtropical Agriculture, Chinese Academy of Sciences [2017QNCXTD_GTD]
- Dept. Premier and Cabinet, Office of Science
- University of Western Australia
Carbon monoxide (CO)-oxidizing bacteria are active consumers of atmospheric CO, undergoing a CO oxidation process that is catalyzed by carbon monoxide dehydrogenase (CODH). Little is known about the phylogeny of a group of bacterial CO-oxidizers known as the BMS Glade, which possess putative CODH genes, or their abundances in soils. In this study coxL genes indicative of bacteria belonging to the BMS Glade were amplified from DNA directly extracted from four vegetated soils: paddy rice (PR) soil, maize cropland (ML) soil, tea plantation (TP) soil, and natural forest (NF) soil. Quantitative PCR (qPCR) analysis showed that BMS coxL gene copy numbers ranged from 1.47 x 10(7) to 7.40 x 10(8) copies g(-1)dry soil and differed significantly among PR, ML, TP, and NF soils (P < 0.05). CoxL gene abundance appeared to be linked to total P and Olsen P concentrations, as revealed by correlation analysis (P < 0.05). Phylogenetic analysis of BMS coxL sequences indicated a diverse Glade of BMS CO oxidizers closely related to Bradyrhizobium sp. ARR65, Rhizobiales GAS188, Paraburkholderia phytofirmans, and Tistlia consotensis. However, the community compositions of BMS coxL-containing bacteria differed among soils. Collectively, these results indicate a high abundance and diversity of BMS Glade CO oxidizers in a wide variety of vegetated soils. (C) 2017 Elsevier Masson SAS. All rights reserved.
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