Isolation and characterization of two highly insecticidal, endophytic strains of Bacillus thuringiensis

Recent discovery of endophytic strains of Bacillus thuringiensis significantly improves the knowledge on its ecology. It also may be a new source for the isolation of insecticidal strains. This report shows the characterization of two endophytic, highly insecticidal strains of B. thuringiensis. Strains LBIT-1250L and LBIT-1251P were isolated from lavender and Poinsettia sap, respectively. Their parasporal crystals were very similar in morphology to those shown by serotypes israelensis and kurstaki, respectively. Bioassays on Aedes aegypti fourth instar larvae and on Manduca sexta first instar larvae, respectively, showed significantly higher levels of toxicity than those of their standard counterparts, IPS-82 (israelensis) and HD-1 (kurstaki) strains, respectively. Characterization of both strains included the sequencing of flagellin (hag) gene, plasmid and Bc Rep-PCR patterns and crystal protein content. All four characterization features indicated that LBIT1250L is highly related to the IPS-82 standard (serotype H-14: israelensis); while the LBIT-1251P was highly related to the HD-1 standard (serotype H-3a3b3c kurstaki). These results indicate that endophytic strains of B. thuringiensis may be a new source of potential insecticidal strains and opens more in-depth studies about the role of this bacterium in such a specialized habitat.

Automated summary

The recent discovery of endophytic strains of Bacillus thuringiensis has greatly enhanced understanding of its ecology and provided a potential new source of insecticidal strains. Two endophytic strains of B. thuringiensis isolated from lavender and Poinsettia sap exhibited high insecticidal activity, similar morphology, and increased toxicity compared to standard strains. Characterization of these strains indicated their relationship to existing standard serotypes and the potential of endophytic strains as a valuable resource for insecticidal applications.