期刊
LWT-FOOD SCIENCE AND TECHNOLOGY
卷 150, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.lwt.2021.112023
关键词
Microalgae; Nutritional quality; Amylase; Glucosidase; Molecular docking
资金
- Y Shiraz University [GR-AGRST108]
Microalgae proteins are new sources of functional amino acids with nutritional value comparable to conventional protein sources, and their amino acid extracts exhibit anti-amylase and anti-glucosidase properties. Kinetic and molecular docking analyses show that these amino acids inhibit enzyme activity through non-competition and un-competition strategies.
Since microalgae proteins contain the same amount of protein as conventional protein sources such as soybean, milk, beef, and egg, they are considered as new sources of functional amino acids. In this research, microalgae amino acid extracts were studied for their nutritional value as well as their anti-amylase and anti-glucosidase properties, in vitro and in silico. Essential, non-essential, non-protein, flavor, hydrophobic, aromatic, bitter, and sweet amino acids were found to be equal to or greater than those found in egg protein in the selected microalgae. The anti-amylase and anti-glucosidase properties of amino acid extract were confirmed in this study, but at a lower level than acarbose. Microalgae amino acids inhibited amylase and glucosidase by increasing the K-m/V-max ratio through non-competition and un-competition strategy, according to the kinetic analysis. Molecular docking analysis revealed that microalgae amino acids interact with amino acid residues near the active site of amylase and allosteric sites of glucosidase through p-alkyl interactions, positive-negative interactions, salt bridges, and hydrogen bonds. In conclusion microalgae amino acid not only can be considered as functional food with nutritional quality equal to egg protein, but also due to functional amino acid can be imperative as noncompetitive and un-competitive inhibitor of amylase and glucosidase.
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