期刊
STEM CELLS
卷 39, 期 12, 页码 1733-1750出版社
OXFORD UNIV PRESS
DOI: 10.1002/stem.3443
关键词
AhR; cell differentiation; epidermal stem cells; hair follicle; quiescence; regeneration; skin homeostasis
资金
- Consejeria de Economia, Ciencia y Agenda Digital, Junta de Extremadura [GR18006, IB160210, IB20014]
- Ministerio de Ciencia e Innovacion [PID2020-114846RB-I00, SAF2017-82597-R]
The aryl hydrocarbon receptor (AhR) plays a crucial role in skin regeneration and homeostasis by maintaining the identity of epidermal stem cells, making it a potential target for treating cutaneous pathologies.
Skin integrity requires constant maintenance of a quiescent, yet responsive, population of stem cells. While interfollicular epidermal progenitors control normal homeostasis, hair follicle stem cells residing within the bulge provide regenerative potential during hair cycle and in response to wounding. The aryl hydrocarbon receptor (AhR) modulates cell plasticity and differentiation and its overactivation results in severe skin lesions in humans. However, its physiological role in skin homeostasis and hair growth is unknown. Reconstitution assays grafting primary keratinocytes and dermal fibroblasts into nude mice and 3-D epidermal equivalents revealed a positive role for AhR in skin regeneration, epidermal differentiation, and stem cell maintenance. Furthermore, lack of receptor expression in AhR-/- mice delayed morphogenesis and impaired hair regrowth with a phenotype closely correlating with a reduction in suprabasal bulge stem cells (alpha 6(low)CD34(+)). Moreover, RNA-microarray and RT-qPCR analyses of fluorescence-activated cell sorting (FACS)-isolated bulge stem cells revealed that AhR depletion impaired transcriptional signatures typical of both epidermal progenitors and bulge stem cells but upregulated differentiation markers likely compromising their undifferentiated phenotype. Altogether, our findings support that AhR controls skin regeneration and homeostasis by ensuring epidermal stem cell identity and highlights this receptor as potential target for the treatment of cutaneous pathologies.
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