期刊
BIORESOURCES AND BIOPROCESSING
卷 8, 期 1, 页码 -出版社
SPRINGER HEIDELBERG
DOI: 10.1186/s40643-021-00426-x
关键词
Porphyridium purpureum; Nitrogen deprivation; Transcriptome analysis; Exopolysaccharides; Polyunsaturated fatty acids; Phycoerythrin
资金
- National Key Research and Development Project of China [2019YFA0906300, 2020YFA0907304]
- Natural Science Foundation of Shandong Province [ZR2019ZD17]
- National Natural Science Foundation of China [31872608]
- Natural Science Foundation of Shanghai [21ZR1416400, 18ZR1410100]
- Shanghai Pujiang Program [18PJD008]
- Funding Project of the State Key Laboratory of Bioreactor Engineering
Porphyridium purpureum is a mesophilic, unicellular red alga rich in phycoerythrin, sulfate polysaccharides, and polyunsaturated fatty acids. Nitrogen deficiency inhibits its growth, resulting in cell yellowing and thickening of the extracellular viscous sheath. This study shows that under nitrogen stress, P. purpureum increases the content of total lipids and exopolysaccharides.
Porphyridium purpureum is a mesophilic, unicellular red alga rich in phycoerythrin, sulfate polysaccharides, and polyunsaturated fatty acids. Nitrogen deficiency inhibited the growth of P. purpureum and resulted in yellowing of the cells and thickening of the extracellular viscousness sheath. Under nitrogen stress, the contents of total lipids and exopolysaccharides in P. purpureum were increased by 65.2% and 188.0%, respectively. We demonstrate that the immediate response of P. purpureum to nitrogen deficiency is mediated by carbon flow to polysaccharide synthesis, while the synthesis of lipids is enhanced as a permanent energy storage substance at the later stage. Based on transcriptome annotation information, we elucidate the synthesis pathway of polysaccharides from P. purpureum from the perspective of glycosyl-donor interconversion, and demonstrate that the n-6 pathway is the main synthesis pathway of polyunsaturated fatty acids. This study not only provides a production strategy for polysaccharides and fatty acids by single-celled marine red algae P. purpureum, but also provides targets for further genetic modification.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据