期刊
NANOSCALE
卷 13, 期 37, 页码 15552-15559出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d1nr02757c
关键词
-
类别
资金
- National Natural Science Foundation of China [61873037, 61903039]
- China Postdoctoral Science Foundation [BX20190035, 2020M680015]
- National Science Foundation USA [DMR-1827346, JP19H02093, JP19H02097]
- Ministry of Education, Culture, Sports, Science and Technology of Japan
The article discusses a stable tetrahedral DNA nanorobot that can undergo controlled conformational changes in response to the stimulation of a specific molecular biomarker, EpCAM. The nanorobot shows low cytotoxicity and target specificity, making it a promising tool for precise monitoring of EpCAM-positive cells.
Dynamic DNA origami nanostructures that respond to external stimuli are promising platforms for cargo delivery and nanoscale sensing. However, the low stability of such nanostructures under physiological conditions presents a major obstacle for their use in biomedical applications. This article describes a stable tetrahedral DNA nanorobot (TDN) programmed to undergo a controlled conformational change in response to epithelial cell adhesion molecule (EpCAM), a molecular biomarker specifically expressed on the circulating tumor cells. Multiresolution molecular dynamics simulations verified the overall stability of the folded TDN design and characterized local distortions in the folded structure. Atomic force microscopy and gel electrophoresis results showed that tetragonal structures are more stable than unfolded DNA origami sheets. Live cell experiments demonstrated the low cytotoxicity and target specificity of TDN. In summary, the proposed TDN can not only effectively resist nuclease catalysis but also has the potential to monitor EpCAM-positive cells precisely.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据