期刊
CHEMICAL COMMUNICATIONS
卷 57, 期 71, 页码 8977-8980出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d1cc03743a
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This study presents a novel photoelectrochemical biosensor using CRISPR/Cas12a system for the quantification of HPV-related DNA by cleaving dopamine-loaded liposome-encoded magnetic beads.
This study reports a photoelectrochemical biosensor for dopamine-loaded liposome-encoded magnetic beads cleaved by clustered regularly interspaced short palindromic repeats (CRISPR)/Cas 12a system for the quantification of human papilloma virus (HPV)-related DNA using neodymium-doped BiOBr nanosheets (Nd-BiOBr) as a photoactive matrix. Magnetic beads and dopamine-loaded liposomes are covalently attached to the both ends of ssDNA to construct dumbbell-shaped dopamine-loaded liposome-encoded magnetic bead (DLL-MB) probes. When the guide RNA binds to the target HPV-16, the ssDNA will be cleaved by Cas12a, thereby degrading the double dumbbell probes. After magnetic separation, the dissolved DLLs are treated with Triton X-100 to release the dopamine (as an electron donor), which was then detected by an amplified photocurrent using the Nd-BiOBr-based photoelectrode.
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