期刊
EUROPEAN JOURNAL OF PROTISTOLOGY
卷 60, 期 -, 页码 45-49出版社
ELSEVIER GMBH
DOI: 10.1016/j.ejop.2017.05.008
关键词
Mamiellophyceae; Picoplankton; Ribosomal genes; Southern Patagonia
类别
资金
- FONCYT of Argentina [PICT 32732, 0794]
- CONICET of Argentina [PIP 498]
- Swiss National Science Foundation project [FN 31003A-143960/1]
- CONICYT from Chile [21110037, 78130011]
We characterized molecularly the first freshwater member ever reported for the family Bathycoccaceae in Lake Musters (Argentinean Patagonia). Members of this family are extremely numerous and play a key ecological role in marine systems as primary producers. We cloned a fragment comprising the SSU rRNA gene + ITS region from environmental DNA using specific mamiellophyte primers. The unique SSU rRNA gene sequence obtained clustered robustly with Bathycoccus prasinos. Analysis of the two-dimensional structure of the ITS region showed the presence of a typical supplementary helix in the ITS-2 region, a synapomorphy of Bathycoccaceae, which confirmed further its phylogenetic placement. We finally discuss the possible causes for the presence of this organism in Lake Musters. (C) 2017 Elsevier GmbH. All rights reserved.
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