期刊
EUROPEAN JOURNAL OF PLANT PATHOLOGY
卷 148, 期 4, 页码 853-866出版社
SPRINGER
DOI: 10.1007/s10658-016-1141-1
关键词
Concatenated analysis; Foliar nematodes; Molecular barcoding; Phylogeny; rDNA
资金
- PEACE Project (Erasmus Mundus)
- University of Costa Rica (UCR)
- Ministerio de Ciencia, Tecnologia y Telecomunicaciones (MICITT)
- Consejo Nacional para Investigaciones Cientificas y Tecnologicas (CONICIT)
- special research fund UGent [01 N02312]
Composed mostly of fungivorous species, the genus Aphelenchoides also comprises 14 plant-parasitic species. The most common and devastating, A. besseyi, A. fragariae, A. ritzemabosi and A. subtenuis have been reported on more than 900 plant species. The combination of low inter-specific and high intra-specific morphological variability makes morphology-based identification extremely difficult within this genus, and has led to molecular tools being employed to ensure accurate diagnoses. rDNA markers are widely used for the identification of nematodes while the Cytochrome Oxidase I gene (COI) remains relatively unexplored despite its role as the standard barcode for almost all animal groups. To explore its suitability as a diagnostic tool, we studied a fragment of the mtCOI region of the four main plant-parasitic Aphelenchoides within a phylogenetic framework. We generated 69 mtCOI and 123 rDNA sequences of diverse Aphelenchoides taxa; 67 belong to the main plant-parasitic species including the first mtCOI sequence of A. fragariae and the first mtCOI and 28S sequences of A. subtenuis. mtCOI had a similar success rate for PCR amplification. Phylogenetic trees based on the three studied markers are largely in agreement with one another, validating their use for Aphelenchoides diagnosis; additionally, we were able to locate several misidentified sequences of plant-parasitic Aphelenchoides in existing databases. The concatenated analysis from the three markers resulted in a more robust insight into the phylogeny and evolution of Aphelenchoides, revealing that plant-parasitism has evolved independently at least three times within this genus, presumably from fungal-feeding ancestors.
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