期刊
JOURNAL OF TISSUE ENGINEERING
卷 12, 期 -, 页码 -出版社
SAGE PUBLICATIONS INC
DOI: 10.1177/20417314211031378
关键词
ROS-scavenging hydrogels; MSCs; skeletal muscle; regeneration
资金
- National Natural Science Foundation of China [81873995, 81974334]
- Preponderant Discipline Supporting Project of the Second Affiliated Hospital of Soochow University [XKTJ-XK202003]
- Suzhou Special Foundation for the Key Diseases Diagnosis and Treatment [LCZX201904, LCZX201708]
- Key Laboratory for Spinal Cord Injury Regeneration of Suzhou [SZS201807]
- Social Development Key Programs of Jiangsu Province-Advanced Clinical Technology [BE2019662, BE2018656]
- Postgraduate Research & Practice Innovation Program of Jiangsu Province [KYCX19_1983, KYCX20_2678]
- Advanced Ph.D. research project of the Second Affiliated Hospital of Soochow University [SDFEYBS2011]
The study demonstrated that a novel hydrogel loaded with mesenchymal stem cells could reduce skeletal muscle damage, promote regeneration, and improve motor function by enhancing the polarization of M2 macrophages and increasing protein expression.
Skeletal muscle injury is a common disease accompanied by inflammation, and its treatment still faces many challenges. The local inflammatory microenvironment can be modulated by a novel ROS-scavenging hydrogel (Gel) we constructed. And MSCs could differentiate into myoblasts and contribute to muscle tissue homeostasis and regeneration. Here, Gel loaded with mesenchymal stem cells (MSCs) (Gel@MSCs) was developed for repairing the injured skeletal muscle. Results showed that the Gel improved the survivability and enhanced the proliferation of MSCs (approximate to two-fold), and the Gel@MSCs inhibited the local inflammatory responses as it promoted polarization of M2 macrophages (increased from 5% to 17%), the mediator of the production of anti-inflammatory factors. Western blotting and qPCR revealed the Gel promoted the expression of proteins (approximate to two-fold) and genes (approximate to two to six-fold) related to myogenesis in MSCs. Histological assessment indicated that the Gel or MSCs promoted regeneration of skeletal muscle, and the efficacy was more significant at Gel@MSCs than MSCs alone. Finally, behavioral experiments confirmed that Gel@MSCs improved the motor function of injured mice. In short, the Gel@MSCs system we constructed presented a positive effect on reducing skeletal muscle damage and promoted skeletal muscle regeneration, which might be a novel treatment for such injuries.
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