期刊
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
卷 117, 期 -, 页码 324-332出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ejpb.2017.05.002
关键词
Stimuli-responsive nanogels; Dextran; Cell uptake; Controlled drug release; Flow cytometry; Tumor cells
资金
- Spanish Ministry of Economy, Industry and Competitiveness (MINECO) [SAF2014-52632-R]
- FEDER (Spain)
- University of Calabria (Italy)
- MINECO [BES-2012-051889]
pH/redox dual-responsive nanogels (DEX-SS) were prepared by precipitation polymerization of methacrylated dextran (DEXMA), 2-aminoethylmethacrylate (AEMA) and N,N'-bis(acryloyl)cystamine (BAC), and then loaded with methotrexate (MTX). Nanogels were spherical and exhibited homogeneous size distribution (460 nm, PDI < 0.30) as observed using dynamic light scattering (DLS) and scanning electron microscopy (SEM). DEX-SS were sensitive to the variations of pH and redox environment. Nanogels incubated in buffer pH 5.0 containing 10 mM glutathione (GSH) synergistically increased the mean diameter and the PDI to 750 nm and 0.42, respectively. In vitro release experiments were performed at pH 7.4 and 5.0 with and without GSH. The cumulative release of MTX in pH 5.0 medium with 10 mM GSH was 5 fold higher than that recorded at pH 7.4 without GSH. Fibroblasts and tumor cells were used to tests the effects of blank DEX-SS and MTX@DEX-SS nanogels on cell viability. Remarkable influence of pH on nano gels internalization into HeLa cells was evidenced by means of confocal microscopy and flow cytometry. (C) 2017 Elsevier B.V. All rights reserved.
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