期刊
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
卷 100, 期 -, 页码 126-131出版社
ELSEVIER
DOI: 10.1016/j.ejps.2017.01.019
关键词
Venous malformations; TGF-beta; miR-145; Bleomycin A5
资金
- National Natural Science Foundation of China [81371159, 81300895, 81570994, 81600385]
Venous malformations (VMs) are characterized by ectatic and tortuous venous channels with decreased perivascular cell coverage. Recent studies have discovered that miR-145 plays a critical role in amounts of vascular diseases through regulating the differentiation and phenotype of vascular smooth muscle cells (VSMCs). However, the potential roles of miR-145 in VMs remain unknown. In this study, 21 samples of VMs without treatment history, and 10 samples of healthy donor skin, were collected to evaluate the expression level of TGF-beta, miR-145, and alpha-SMA by immunohistochemistry, in situ hybridization, and real-time polymerase chain reaction (PCR). Subsequently, their correlations were analyzed using the Spearman rank correlation testin vitro studies were performed using human umbilical vein endothelial cells (HUVECs). The results showed that miR-145 was significantly downregulated in VMs compared with normal skin tissues, accompanied by a synchronously decreased TGF-beta expression level and perivascular alpha-SMA(+) cell coverage. Correlation analysis revealed that miR145 expression was positively correlated with TGF-beta expression and perivascular alpha-SMA+ cell coverage in VMs. In addition, TGF-beta, miR-145, and alpha-SMA were concurrently increased in the tissues of VMs treated with bleomycin A5. More importantly, in vitro studies revealed that both recombinant human TGF-beta and bleomycin A5 could significantly upregulate TGF-beta and miR-145 expression in HUVECs with the similar increasing tendency. In summary, our present study unmasked the downregulation of miR-145 in VMs, possibly induced by TGF-beta depression and closely correlated with disorganized vessels. Moreover, miR-145 may be involved in the sclerotherapy of VMs and possess the target potential. (C) 2017 Elsevier B.V. All rights reserved.
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