期刊
SENSORS AND ACTUATORS REPORTS
卷 3, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.snr.2021.100033
关键词
Isothermal nucleic acid amplification reactions; Molecular detection; Diagnostics; Enzyme stability; Sensitivity; Specificity; Nonspecific amplification
类别
资金
- National Science Foundation [1847245]
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [1847245] Funding Source: National Science Foundation
In this review, various reaction additives and techniques to enhance the sensitivity, specificity, stability, robustness, or speed of isothermal nucleic acid amplification reactions for sensing applications are summarized. The mechanisms and purposes of each enhancement technique are provided to guide the selection of reaction additives to optimize novel isothermal amplification systems and assays.
Isothermal nucleic acid amplification techniques are used to detect a variety of molecules such as RNAs, DNAs and proteins. When compared to the gold standard polymerase chain reaction, isothermal methods are rapid and simple but often have lower specificity and sensitivity. In this review, we provide a summary of reaction additives and techniques that enhance the sensitivity, specificity, stability, robustness, or speed of isothermal nucleic acid amplification reactions used for sensing applications. We also provide the mechanism and purpose of each enhancement technique, which can provide guidance when selecting reaction additives to optimize novel isothermal amplification systems and assays.
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