Assessing the Potential of the Terrestrial Cyanobacterium Anabaena minutissima for Controlling Botrytis cinerea on Tomato Fruits

Cyanobacteria are oxygenic phototrophs that have an essential role in soil N-2 fixation, fertility, and water retention. Cyanobacteria are also natural sources of bioactive metabolites beneficial to improve plant vigor and potentially active against fungal plant pathogens. Therefore, we studied the antifungal activity of water extract (WE) and phycobiliproteins (PBPs) from Anabaena minutissima strain BEA 0300B against the fungal plant pathogen Botrytis cinerea on tomato fruits and in vitro. The water extract and PBPs were characterized by using FT-IR and FT-Raman spectroscopies. Both water extract (5 mg/mL) and PBPs (ranged from 0.3 to 4.8 mg/mL) reduced disease incidence and disease severity on tomato fruits and mycelium growth and colony forming units in vitro. For mycelium growth, a linear PBP dose-response was found. Tomato fruits were also characterized by FT-IR and FT-Raman spectroscopies in order to evaluate structural modifications induced by pathogen and PBP treatment. PBPs preserved cutin and pectin structures by pathogen challenge. In conclusion, A. minutissima can be considered a potential tool for future large-scale experiments for plant disease control.

Automated summary

Cyanobacteria play important roles in soil nitrogen fixation, fertility, and water retention, and produce bioactive metabolites beneficial for plants against fungal pathogens. Water extract and phycobiliproteins from Anabaena minutissima showed antifungal activity against Botrytis cinerea, reducing disease severity in tomato fruits and in vitro. The study indicated that these compounds could potentially be used for plant disease control in the future.