期刊
HORTICULTURAL PLANT JOURNAL
卷 7, 期 5, 页码 434-442出版社
ELSEVIER
DOI: 10.1016/j.hpj.2021.05.005
关键词
m(6)A methylation; Chilling injury; Plant hormone; Nanopore direct RNA sequencing; Tomato fruit
资金
- National Natural Science Foundation of China [31772022, 32072284, 31501544]
- Special Innovation Ability Construction Fund of Beijing Academy of Agricultural and Forestry Sciences [20200427, 20210437]
- Science and Technology Planning Project of Tianjin City [19YFSLQY00100]
- Beijing Municipal Science and Technology Commission [Z191100008619004, Z191100004019010]
- China Agriculture Research System of MOF and MARA
- collaborative innovation center of Beijing Academy of Agricultural and Forestry Sciences [201915]
- Young Investigator Fund of Beijing Academy of Agricultural and Forestry Sciences [202016]
- key scientific research projects of colleges and universities in Henan Province [20A550014]
The study identified a relationship between chilling injury in tomato fruit and N-6-methyladenosine (m(6)A) methylation of transcripts in plant hormone pathways, involving various enzymes and transcription factors. The findings provide a deeper understanding of the regulatory mechanism of chilling injury and post-harvest cold storage of tomato fruit.
Tomato fruit are sensitive to chilling injury (CI) during cold storage. Several factors have been discovered to be involved in chilling injury of tomato fruit. Plant hormones play an important regulatory role, however, the relationship between chilling injury and N-6-methyladenosine (m(6)A) methylation of transcripts in plant hormone pathways has not been reported yet. In order to clarify the complex regulatory mechanism of m(6)A methylation on chilling injury in tomato fruit, Nanopore direct RNA sequencing was employed. A large number of enzymes and transcription factors were found to be involved in the regulation process of fruit chilling injury, which were associated with plant hormone, such as 1-aminocyclopropane 1-carboxylate synthase (ACS ), aspartate aminotransferase (AST ), auxin response factor (ARF2), ethylene response factor 2 (ERF2), gibberellin 20-oxidase-3 (GA20ox) and jasmonic acid (JA ). By conjoint analysis of the differential expression transcripts related to chilling injury and m6A methylation differential expression transcripts 41 differential expression transcripts were identified involved in chilling injury including 1-aminocyclopropane-1-carboxylate oxidase (ACO) and pectinesterase (PE ) were down-regulated and heat shock cognate 70 kD protein 2 (cpHSC70), HSP70-binding protein (HspBP) and salicylic acid-binding protein 2 (SABP2) were up-regulated. Our results will provide a deeper understanding for chilling injury regulatory mechanism and post-harvest cold storage of tomato fruit.
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