4.8 Article

Clustering of catalytic nanocompartments for enhancing an extracellular non-native cascade reaction

期刊

CHEMICAL SCIENCE
卷 12, 期 37, 页码 12274-12285

出版社

ROYAL SOC CHEMISTRY
DOI: 10.1039/d1sc04267j

关键词

-

资金

  1. Swiss National Science Foundation
  2. University of Basel
  3. National Centre of Competence in Research - Molecular Systems Engineering

向作者/读者索取更多资源

Compartmentalization and controlled distance between catalytic nanocompartments play a crucial role in regulating cascade reactions. Utilizing DNA hybridization to cluster enzyme-loaded polymersomes significantly enhances the efficiency of spatially segregated enzymes in cascade reactions. Interaction of clusters with cell surfaces, mediated by residual DNA single strands, leads to non-native enzymatic cascades in cells. The self-organization into clusters of catalytic nanocompartments offers new possibilities for highly efficient applications in catalysis and nanomedicine.
Compartmentalization is fundamental in nature, where the spatial segregation of biochemical reactions within and between cells ensures optimal conditions for the regulation of cascade reactions. While the distance between compartments or their interaction are essential parameters supporting the efficiency of bio-reactions, so far they have not been exploited to regulate cascade reactions between bioinspired catalytic nanocompartments. Here, we generate individual catalytic nanocompartments (CNCs) by encapsulating within polymersomes or attaching to their surface enzymes involved in a cascade reaction and then, tether the polymersomes together into clusters. By conjugating complementary DNA strands to the polymersomes' surface, DNA hybridization drove the clusterization process of enzyme-loaded polymersomes and controlled the distance between the respective catalytic nanocompartments. Owing to the close proximity of CNCs within clusters and the overall stability of the cluster architecture, the cascade reaction between spatially segregated enzymes was significantly more efficient than when the catalytic nanocompartments were not linked together by DNA duplexes. Additionally, residual DNA single strands that were not engaged in clustering, allowed for an interaction of the clusters with the cell surface as evidenced by A549 cells, where clusters decorating the surface endowed the cells with a non-native enzymatic cascade. The self-organization into clusters of catalytic nanocompartments confining different enzymes of a cascade reaction allows for a distance control of the reaction spaces which opens new avenues for highly efficient applications in domains such as catalysis or nanomedicine.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.8
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据