期刊
JOURNAL OF THE ROYAL SOCIETY INTERFACE
卷 18, 期 182, 页码 -出版社
ROYAL SOC
DOI: 10.1098/rsif.2021.0181
关键词
Biogran; osseointegration; biointerphase; scanning transmission electron microscopy; electron tomography; focused ion beam
资金
- CAPES (UNESP/CAPES/Print Project)
- Sao Paulo Research Foundation (FAPESP) [2017/08187-3]
- Natural Sciences and Engineering Research Council of Canada (NSERC) [RGPIN-2020-05722]
- OntarioMinistry of Research, Science and Innovation [ER17-13081]
- Canada Research Chairs Programme
This paper presents a workflow for characterizing the interface between newly formed bone and bioglass at the nanoscale, utilizing scanning and transmission electron microscopy techniques. The study provides unprecedented resolution of the bone-BG interface and proposes a method for three-dimensional visualization using electron tomography.
The success of biomaterials for bone regeneration relies on many factors, among which osseointegration plays a key role. Biogran (BG) is a bioactive glass commonly employed as a bone graft in dental procedures. Despite its use in clinical practice, the capability of BG to promote osseointegration has never been resolved at the nanoscale. In this paper, we present the workflow for characterizing the interface between newly formed bone and BG in a preclinical rat model. Areas of bone-BG contact were first identified by backscattered electron imaging in a scanning electron microscope. A focused ion beam in situ lift-out protocol was employed to prepare ultrathin samples for transmission electron microscopy analysis. The bone-BG gradual interface, i.e. the biointerphase, was visualized at the nanoscale with unprecedented resolution thanks to scanning transmission electron microscopy. Finally, we present a method to view the bone-BG interface in three dimensions using electron tomography.
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