Differential Pulse Voltammetric Strategy for Simultaneous Determination of Aceclofenac and Ezogabine in Biological Fluids at Edge-plane Pyrolytic Graphite Electrode

Herein, an eco-friendly and rapid voltammetric method is suggested for simultaneous assay of Aceclofenac (ACL) and Ezogabine (EZO) in biological fluids. Aceclofenac (ACL) and Ezogabine (EZO) are co-administrative drugs which are prescribed for the treatment of epilepsy associated with chronic inflammatory. The voltammetric method is based on the electrochemical oxidation of ACL and EZO at an edge plane pyrolytic graphite electrode using differential pulse voltammetry (DPV). Moreover, the electrochemical oxidation at carbon paste electrode and carbon paste electrodes modified with nanoparticles have been remarked. A number of crucial parameters which affect the electrochemical process at the electrode surface have been optimized. The method is linear over the concentration ranges from 0.2 to 100.0 mu M and from 2.0 to 70.0 mu M for ACL and EZO, respectively. The average recovery was found to be 100 +/- 2% with satisfactory %RSD (<1.5%) for both compounds. The method was applied successfully for simultaneous determination of ACL and EZO in biological samples with satisfactory accuracy and precision.

Automated summary

An eco-friendly and rapid voltammetric method was suggested for simultaneous assay of Aceclofenac and Ezogabine in biological fluids. The method showed good linearity, recovery rate, and was successfully applied for simultaneous determination of the two compounds in biological samples.