期刊
EUROPEAN JOURNAL OF ORAL SCIENCES
卷 125, 期 6, 页码 438-443出版社
WILEY
DOI: 10.1111/eos.12387
关键词
apoptotic cell death; DNA damage; oral cancer; oridonin; phosphorylation of H2AX
资金
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Science ICT & Future Planning [2016R1A2B4006794, 2017R1D1A1B03029124]
- National Research Foundation of Korea [2016R1A2B4006794, 2017R1D1A1B03029124] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Oridonin, a natural diterpenoid purified from Rabdosia rubescens, has displayed beneficial biological activities, including anti-proliferation and anti-angiogenesis effects, in various types of cancers. However, the anti-cancer potential of oridonin and its mechanism in oral cancer have never previously been studied. In this study, we assessed the role of oridonin as an inducer of apoptosis in HSC-3 and HSC-4 human oral cancer cells. Our results showed that oridonin reduces the viability of human oral cancer cells and significantly increases the expression of H2AX, a well-known marker of DNA damage. 4,6-Diamidino-2-phenylindole (DAPI) staining and western blotting showed that oridonin causes nuclear condensation and fragmentation, and induces cleavage of poly(ADP-ribose) polymerase (PARP). Moreover, oridonin-induced H2AX accumulation was partially abrogated by Z-VAD, a pan-caspase inhibitor. Taken together, our results suggest that oridonin can effectively induce apoptosis by augmenting the expression of H2AX in response to DNA damage and might be a promising anti-cancer drug candidate for the treatment of oral cancer.
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