Doubled haploid production in advanced back cross generations and molecular cytogenetic characterization of rye chromatin in triticale x wheat derived doubled haploid lines

The rye genome has shown potential for improvement of bread wheat, where wheat-rye substitutions and translocations have been and are frequently used in resistance breeding. Crosses belongs to different generations viz., BC1F1, BC1F2, BC1F3, BC1F4 and BC2F3 of triticale x wheat derived were used for different haploid induction parameters using Gogon grass (Imperata cylindrica) as a pollen source. The percentage of pseudo seed formation ranged from 34.55% for BC1F2 to 63.77 for BC1F1 crosses, the haploid embryo formation ranges from 9.43% for BC1F1 to 30.2% for BC1F2, the haploid plant generation ranges from 19.36% for BC1F2 to 63.25% for BC1F1. Four doubled haploids were developed from ITSN 105/58 x VL 802 x VL 802 of BC2F3 underwent molecular cytogenetic analyses using the probes, viz., rye genomic rDNA, pSc 119 and pAs1. FISH and GISH analysis revealed an IBL.1RS translocation and substitution of 5R chromosome instead of the 5D chromosomes in these doubled haploids.

Automated summary

The study explores the potential of improving bread wheat using the rye genome, with wheat-rye substitutions and translocations being commonly utilized for resistance breeding. Crosses of different generations were used to assess various haploid induction parameters, and molecular cytogenetic analysis revealed an IBL.1RS translocation and substitution of the 5R chromosome in doubled haploids from BC2F3.