Detection of chromosome instability by interphase FISH in mouse and human tissues

Chromosomal instability (CIN), a type of genomic instability, favors changes in chromosome number and structure and it is associated with the progression and initiation of multiple diseases, including cancer. Therefore, CIN identification and analysis represents a useful tool for cancer diagnosis and treatment. Here, we report an optimized molecular cytogenetic protocol to detect CIN in formalin-fixed, paraffin-embedded mouse and human tissues, using fluorescent in situ hybridization to visualize and quantify chromosomal alterations such as amplifications, deletions, and translocations. For complete information on the generation and use of this protocol, please refer to Brandt et al. (2018).

Automated summary

Chromosomal instability (CIN) is a type of genomic instability that is associated with the progression of various diseases, including cancer. An optimized molecular cytogenetic protocol has been developed to detect and analyze CIN, providing a useful tool for cancer diagnosis and treatment.