期刊
STAR PROTOCOLS
卷 2, 期 3, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.xpro.2021.100731
关键词
-
资金
- NASPGHAN Foundation Young Investigator Award
- COCVD Pilot and Feasibility Grant
This study presents a luminescence-based protocol to quantify KHK activity, which has been validated for accuracy and specificity through experiments involving knockdown and overexpression of KHK in vivo and in vitro.
Ketohexokinase (KHK) catalyzes the first step of fructose metabolism. Inhibitors of KHK enzymatic activity are being evaluated in clinical trials for the treatment of non-alcoholic fatty liver disease (NAFLD) and diabetes. Here, we present a luminescence-based protocol to quantify KHK activity. The accuracy of this technique has been validated using knockdown and overexpression of KHK in vivo and in vitro. The specificity of the assay has been verified using 3-O-methyl-D fructose, a non-metabolizable analog of fructose, heat inactivation of hexokinases, and depletion of potassium.For complete details on the use of this protocol, please refer to Damen et al. (2021).
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据