Protocol Protocols for endothelial cell isolation from mouse tissues: brain, choroid, lung, and muscle

Endothelial cells (ECs) harbor distinct phenotypical and functional characteristics depending on their tissue localization and contribute to brain, eye, lung, and muscle diseases such as dementia, macular degeneration, pulmonary hyperten-sion, and sarcopenia. To study their function, isolation of pure ECs in high quan-tities is crucial. Here, we describe protocols for rapid and reproducible blood vessel EC purification established for scRNA sequencing from murine tissues us-ing mechanical and enzymatic digestion followed by magnetic and fluorescence -activated cell sorting. For complete details on the use and execution of these protocol, please refer to Kalucka et al. (2020), Rohlenova et al. (2020), and Goveia et al. (2020).

Automated summary

Endothelial cells (ECs) have distinct phenotypical and functional characteristics depending on their tissue localization, playing crucial roles in diseases of various organs. Rapid and reproducible purification of blood vessel ECs can be achieved through mechanical and enzymatic digestion, providing important tools for research.