期刊
STAR PROTOCOLS
卷 2, 期 3, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.xpro.2021.100755
关键词
-
资金
- Council of Scientific and Industrial Research (CSIR) , India [MLP2005]
Sequencing SARS-CoV-2 genomes is crucial for understanding the genetic epidemiology of the COVID-19 pandemic and evolution of the virus, as well as for rapid development of diagnostic tools. The present protocol is a modification of the Illumina COVIDSeq test, describing an amplicon-based next-generation sequencing approach with short turnaround time for bench-top sequencers like MiSeq, iSeq, and Mini-Seq.
Sequencing of SARS-CoV-2 genomes is crucial for understanding the genetic epidemiology of the COVID-19 pandemic. It is also critical for understanding the evolution of the virus and also for the rapid development of diagnostic tools. The present protocol is a modification of the Illumina COVIDSeq test. We describe an amplicon-based next-generation sequencing approach with short turnaround time, adapted for bench-top sequencers like MiSeq, iSeq, and Mini-Seq. For complete details on the use and execution of this protocol, please refer to Bhoyar et al. (2021).
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