期刊
STAR PROTOCOLS
卷 2, 期 3, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.xpro.2021.100779
关键词
-
资金
- JSPS KAKENHI [17K14941]
- OIST Graduate University
ISI is a technique used to map the functional architecture of the cortex based on hemodynamic responses. The protocol described involves using a cranial window with an access port to identify the area of V1 in a head-fixed mouse, followed by targeted viral vector injection for subsequent two-photon imaging of V1 layer 6 corticothalamic neurons. For more details, refer to the paper by Augustinaite and Kuhn (2020b).
Intrinsic optical signal imaging (ISI) is a hemodynamic response-based technique to map the functional architecture of the cortex. ISI is often used as an auxiliary method to localize cortical areas for targeted electrophysiology, pharmacology, or imaging experiments. Here, we provide a protocol for ISI through a cranial window with an access port to identify the area of the primary visual cortex (V1) in a head-fixed mouse, followed by targeted viral vector injection, which enables subsequent two-photon imaging of V1 layer 6 corticothalamic neurons. For complete details on the use and execution of this protocol, please refer to our paper Augustinaite and Kuhn (2020b).
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据