期刊
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
卷 36, 期 1, 页码 1988-1995出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/14756366.2021.1972995
关键词
Carbonic anhydrases; CA classes; hydratase activity; bacteria; catalytic pocket
资金
- Italian Ministry of University and Research [FISR2019_04819]
Carbonic anhydrases (CAs) are a versatile enzyme superfamily involved in multiple physiological processes, and recent discoveries have revealed a new type of iota-CAs with unique catalytic activity without the need for metal ions.
Carbonic anhydrases (CAs, EC 4.2.1.1) have been studied for decades and have been classified as a superfamily of enzymes which includes, up to date, eight gene families or classes indicated with the Greek letters alpha, beta, gamma, delta, zeta, eta, theta, iota. This versatile enzyme superfamily is involved in multiple physiological processes, catalysing a fundamental reaction for all living organisms, the reversible hydration of carbon dioxide to bicarbonate and a proton. Recently, the iota-CA (LCIP63) from the diatom Thalassiosira pseudonana and a bacterial iota-CA (BteCA iota) identified in the genome of Burkholderia territorii were characterised. The recombinant BteCA iota was observed to act as an excellent catalyst for the physiologic reaction. Very recently, the discovery of a novel iota-CAs (COG4337) in the eukaryotic microalga Bigelowiella natans and the cyanobacterium Anabaena sp. PCC7120 has brought to light an unexpected feature for this ancient superfamily: this iota-CAs was catalytically active without a metal ion cofactor, unlike the previous reported iota-CAs as well as all known CAs investigated so far. This review reports recent investigations on iota-CAs obtained in these last three years, highlighting their peculiar features, and hypothesising that possibly this new CA family shows catalytic activity without the need of metal ions.
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