Paper-Based Analytical Device for the On-Site Detection of Nerve Agents

We report a colorimetric paper-based microfluidic device based on an enzyme inhibition assay that allows the on-site detection of nerve agents by sampling and wicking. The sample and reagents are automatically transported through the channel where an enzyme inhibition reaction is conducted, followed by an enzyme-substrate reaction and a color reaction. This device can detect 0.1 mu g/mL of the nerve agent VX in a 2.5 mu L drop and is nerve agent selective and robust against temperature, pH, and several liquids. We confirmed that sampling procedures (dilution and wiping) are applicable to this device. Furthermore, the fabrication procedure is easy, and the cost is at most a few tens of cents. Thus, the present device provides a practical method for the urgent detection of nerve agents in suspected chemical terrorism incidents.

Automated summary

The colorimetric paper-based microfluidic device reported in this study is capable of on-site detection of nerve agents through an enzyme inhibition assay. It offers a high sensitivity and selectivity for detecting nerve agents, while being easy to fabricate and operate at a low cost.