期刊
GUT MICROBES
卷 13, 期 1, 页码 -出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/19490976.2021.1926840
关键词
Human gut microbiota; SARS-CoV-2; 16S rRNA sequencing; clinical study; microbiome; microbiota; COVID-19
资金
- University of Florida Department of Medicine Gatorade Fund
- UF Health Cancer Center funds
- National Institute of Health TL1 Training Grant at the University of Florida [TL1TR001428, UL1TR001427]
- UF Health Cancer Center
The study found significant differences in the fecal microbial composition between COVID-19 patients and controls, as well as between actively infected patients and recovered patients. Certain genera like Campylobacter and Klebsiella were enriched in COVID-19 patients with gastrointestinal symptoms. Additionally, a substantial proportion of COVID-19 patients tested positive for fecal SARS-CoV-2 RNA. Further research is needed to explore the functional impact of SARS-CoV-2 on gastrointestinal health.
To investigate the relationship between intestinal microbiota and SARS-CoV-2-mediated pathogenicity in a United States, majority African American cohort. We prospectively collected fecal samples from 50 SARS-CoV-2 infected patients, 9 SARS-CoV-2 recovered patients, and 34 uninfected subjects seen by the hospital with unrelated respiratory medical conditions (controls). 16S rRNA sequencing and qPCR analysis was performed on fecal DNA/RNA. The fecal microbial composition was found to be significantly different between SARS-CoV-2 patients and controls (PERMANOVA FDR-P = .004), independent of antibiotic exposure. Peptoniphilus, Corynebacterium and Campylobacter were identified as the three most significantly enriched genera in COVID-19 patients compared to controls. Actively infected patients were also found to have a different gut microbiota than recovered patients (PERMANOVA FDR-P = .003), and the most enriched genus in infected patients was Campylobacter, with Agathobacter and Faecalibacterium being enriched in the recovered patients. No difference in microbial community structure between recovered patients and uninfected controls was observed, nor a difference in alpha diversity between the three groups. 24 of the 50 COVID-19 patients (48%) tested positive via RT-qPCR for fecal SARS-CoV-2 RNA. A significant difference in gut microbial composition between SARS-CoV-2 positive and negative samples was observed, with Klebsiella and Agathobacter being enriched in the positive cohort. No significant associations between microbiome composition and disease severity was found. The intestinal microbiota is sensitive to the presence of SARS-CoV-2, with increased relative abundance of genera (Campylobacter, Klebsiella) associated with gastrointestinal (GI) disease. Further studies are needed to investigate the functional impact of SARS-CoV-2 on GI health.
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