期刊
NANOSCALE ADVANCES
卷 3, 期 20, 页码 5890-5899出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d1na00116g
关键词
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资金
- NCI [R01CA253627, U01CA198892]
- Case Comprehensive Cancer Center Support Grant [P30CA043703]
- Shiverick Family Fund
- Alex's Lemonade Stand Foundation
- NIH Interdisciplinary Biomedical Imaging Training Program [T32EB007509]
The use of IONP-induced hyperthermia has a significant impact on tumor-resident immune cell subpopulations and systemic delivery approach showed superior outcomes in reducing both innate and adaptive immune cells within the tumor. Following the depletion of dysfunctional tumor-resident immune cells, treatment with immune checkpoint inhibitors encouraged the repopulation of the tumor with 'fresh' infiltrating innate and adaptive immune cells, resulting in a significant decrease of the tumor cell population.
Iron oxide nanoparticles (IONPs) have often been investigated for tumor hyperthermia. IONPs act as heating foci in the presence of an alternating magnetic field (AMF). It has been shown that hyperthermia can significantly alter the tumor immune microenvironment. Typically, mild hyperthermia invokes morphological changes within the tumor, which elicits a secretion of inflammatory cytokines and tumor neoantigens. Here, we focused on the direct effect of IONP-induced hyperthermia on the various tumor-resident immune cell subpopulations. We compared direct intratumoral injection to systemic administration of IONPs followed by application of an external AMF. We used the orthotopic 4T1 mouse model, which represents aggressive and metastatic breast cancer with a highly immunosuppressive microenvironment. A non-inflamed and 'cold' microenvironment inhibits peripheral effector lymphocytes from effectively trafficking into the tumor. Using intratumoral or systemic injection, IONP-induced hyperthermia achieved a significant reduction of all the immune cell subpopulations in the tumor. However, the systemic delivery approach achieved superior outcomes, resulting in substantial reductions in the populations of both innate and adaptive immune cells. Upon depletion of the existing dysfunctional tumor-resident immune cells, subsequent treatment with clinically approved immune checkpoint inhibitors encouraged the repopulation of the tumor with 'fresh' infiltrating innate and adaptive immune cells, resulting in a significant decrease of the tumor cell population.
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