期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 22, 期 17, 页码 -出版社
MDPI
DOI: 10.3390/ijms22179542
关键词
broomcorn millet; stress; Sporisorium destruens; transcriptome; Panicum miliaceum; smut
资金
- National Industry Technology Projects of Foxtail Millet and Sorghum grant [CARS-06-A26]
- Shaanxi Province Industry Technology Projects of Coarse Cereals grant [NYKJ-2018-YL1]
- Shaanxi Province Key R&D Projects grant [2018TSCXL-NY-03-01]
The study compared the phenotypes, physiological characteristics, and transcriptomes of resistant and susceptible broomcorn millet varieties under stress, revealing physiological changes in infected plants. Significant differences were found in DEGs between resistant and susceptible varieties, with photosynthesis playing a crucial role in both varieties under disease stress.
Broomcorn millet (Panicum miliaceum L.) affected by smut (caused by the pathogen Sporisorium destruens) has reduced production yields and quality. Determining the tolerance of broomcorn millet varieties is essential for smut control. This study focuses on the differences in the phenotypes, physiological characteristics, and transcriptomes of resistant and susceptible broomcorn millet varieties under Sporisorium destruens stress. In diseased broomcorn millet, the plant height and stem diameter were reduced, while the number of nodes increased. After infection, the activities of superoxide dismutase and peroxidase decreased, and malondialdehyde and relative chlorophyll content (SPAD) decreased. Transcriptome analysis showed 514 and 5452 differentially expressed genes (DEGs) in the resistant and susceptible varieties, respectively. The Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of DEGs showed that pathways related to plant disease resistance, such as phenylpropanoid biosynthesis, plant-pathogen interaction, and plant hormone signal transduction, were significantly enriched. In addition, the transcriptome changes of cluster leaves and normal leaves in diseased broomcorn millet were analysed. Gene ontology and KEGG enrichment analyses indicated that photosynthesis played an important role in both varieties. These findings lay a foundation for future research on the molecular mechanism of the interaction between broomcorn millet and Sporisorium destruens.
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