期刊
BIOENGINEERED
卷 12, 期 1, 页码 5607-5619出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/21655979.2021.1967033
关键词
Long non-coding RNA; LINC01296; head and neck squamous cell carcinoma; migration; invasion; prognosis
资金
- Shenzhen Longhua District Medical and Health Institution District Level Scientific Research Project [2021124]
- Construction Funds of Key Medical Disciplines in Longhua District, Shenzhen [MKD202007090212]
In this study, it was found that LINC01296 was highly expressed in HNSCC and associated with poor prognosis in patients. Silencing of LINC01296 inhibited tumor cell migration and invasion, possibly participating in the progression of HNSCC through protein phosphorylation and microtubule-based process regulation.
Long non-coding RNA (lncRNAs) can participate in gene expression regulation. LINC01296 is abnormally expressed in different tumors and promotes tumorigenesis and development. However, the role of LINC01296 in head and neck squamous cell carcinoma (HNSCC) remains not entirely clear. Thus, to explore LINC01296 expression, biological function and potential mechanism in HNSCC, we used GEPIA and GEO database. QRT-PCR was used to detect the knockout efficiency by LINC01296 inhibition with siRNA. Transwell assay was used to detect the migration and invasion capacity of tumor cells. Then enrichment and immunophenotype correlation analyses were carried out to explore the LINC01296 mechanism in HNSCC. To investigate why LINC01296 was up-regulated in HNSCC, DNA methylation analysis was performed using the DiseaseMeth database. LINC01296 expression was notably up-regulated in HNSCC, which was associated with promoter hypomethylation. Also, it was positively correlated with the HNSCC pathological stage and patients with higher LINC01296 expression levels had a poor prognosis. LINC01296 silencing inhibits HNSCC cell migration and invasion. LINC01296 also participate in the HNSCC progression mainly through protein phosphorylation and microtubule-based process regulation. Overall, LINC01296 was highly expressed in HNSCC, promoted tumor cells' migration and invasion, and might be a potential diagnostic and prognostic marker in HNSCC patients.
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