期刊
PHARMACEUTICAL BIOLOGY
卷 59, 期 1, 页码 1216-1232出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/13880209.2021.1970198
关键词
Chinese medicine; type 2 immune response; neuro-immune communication; airway inflammation
资金
- National Natural Science Foundation of China [81774074]
- 3 Years to Accelerate the Development of Chinese Medicine in Shanghai, China [ZY (2018-2020)-FWTX-4016]
The study demonstrates that M-BYF can alleviate experimental asthma by negatively regulating ILC2s and Th9 cells, as well as the VIP-VPAC2 signaling pathway, reducing airway hyperresponsiveness, inflammation, mucus hypersecretion, and collagen deposition in asthmatic mice.
Context Modified BuShenYiQi formula (M-BYF) is derived from BuShenYiQi formula, used for the treatment of allergic asthma. The exact effect and mechanism of M-BYF on the improvement of asthma remain unclear. Objective We investigated the mechanism underlying the therapeutic effect of M-BYF on allergic asthma. Materials and methods The asthma model was established in female BALB/c mice that were sensitized and challenged with ovalbumin (OVA). Mice in the treated groups were orally treated once a day with M-BYF (7, 14 and 28 g/kg/d) or dexamethasone before OVA challenge. Control and Model group received saline. Pathophysiological abnormalities and percentages of lung type 2 innate lymphoid cells (ILC2s) and Th9 cells were measured. Expression levels of type 2 cytokines and transcription factors required for these cells function and differentiation were analysed. Expression of vasoactive intestinal polypeptide (VIP)-VPAC2 signalling pathway-related proteins, and percentages of VIP expressing (VIP+) cells and VPAC2, CD90 co-expressing (VPAC2(+)CD90(+)) cells were detected. Results M-BYF alleviated airway hyperresponsiveness, inflammation, mucus hypersecretion and collagen deposition in asthmatic mice. M-BYF down-regulated percentages of ILC2s and Th9 cells with lower expression of GATA3, PU.1 and IRF4, reduced IL-5, IL-13, IL-9 and VIP production. The decrease in the expression of VIP-VPAC2 signalling pathway and percentages of VIP+ cells, VPAC2(+)CD90(+) cells were observed after M-BYF treatment. The LD50 value of M-BYF was higher than 90 g/kg. Discussion and conclusions M-BYF alleviated experimental asthma by negatively regulating ILC2s and Th9 cells and the VIP-VPAC2 signalling pathway. These findings provide the theoretical basis for future research of M-BYF in asthma patient population.
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