期刊
VETERINARY RESEARCH
卷 52, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s13567-021-00992-0
关键词
miR-125b; bovine mammary epithelial cell; inflammation; NE-kappa B
资金
- Science and Technology research project of Ningxia Higher Education School [NGY2020007]
- National Natural Science Foundation of China [31960652, 32060749]
- Key Research and Development Project (Talent Introduction Project) of Ningxia Hui Autonomous Region [2019BEB04002]
- Introducing Talent Research Project of Ningxia University [030900001926]
Mastitis is a complex inflammatory disease in dairy cows caused by pathogenic infection, and miR-125b plays a crucial role in its occurrence and development. Downregulation of miR-125b can increase inflammatory factors production, while its upregulation can alleviate the inflammatory response.
Mastitis is a complex inflammatory disease caused by pathogenic infection of mammary tissue in dairy cows. The molecular mechanism behind its occurrence, development, and regulation consists of a multi-gene network including microRNA (miRNA). Until now, there is no report on the role of miR-125b in regulating mastitis in dairy cows. This study found that miR-125b expression is significantly decreased in lipopolysaccharide (LPS)-induced MAC-T bovine mammary epithelial cells. Also, its expression is negatively correlated with the expression of NF-kappa B inhibitor interacting Ras-like 2 (NKIRAS2) gene. MiR-125b target genes were identified using a double luciferase reporter gene assay, which showed that miR-125b can bind to the 3' untranslated region (3' UTR) of the NKIRAS2, but not the 3'UTR of the TNF-alpha induced protein 3 (TNFAIP3). In addition, miR-125b overexpression and silencing were used to investigate the role of miR-125b on inflammation in LPS-induced MAC-T. The results demonstrate that a reduction in miR-125b expression in LPS-induced MAC-T cells increases NKIRAS2 expression, which then reduces NF-kappa B activity, leading to low expression of the inflammatory factors IL-6 and TNF-alpha. Ultimately, this reduces the inflammatory response in MAC-T cells. These results indicate that miR-125b is a pro-inflammatory regulator and that its silencing can alleviate bovine mastitis. These findings lay a foundation for elucidating the molecular regulation mechanism of cow mastitis.
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