期刊
PLANT PHYSIOLOGY
卷 186, 期 1, 页码 655-676出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/plphys/kiab054
关键词
-
资金
- DOE Office of Science [DE-SC0012704]
- National Science Foundation [DMR1332208, DMR-1719875]
- NSF [NNCI-2025233]
- National Natural Science Foundation of China [31301349, 30870154, 30901052, 30900087]
- Schwartz Research Fund for Women in Life Sciences
- Agriculture and Food Research Initiative Awards from the USDA National Institute of Food and Agriculture [2018-67013-27418, 2021-67013-33798]
- NSF-IOS [1656321]
- CRDF-GLOBAL U.S.-Ukraine Competition [OISE-9531011]
- Division Of Integrative Organismal Systems
- Direct For Biological Sciences [1656321] Funding Source: National Science Foundation
Addressing the global food security crisis requires the development of high-yielding crops. Research shows that copper deficiency affects grain development in wheat and Brachypodium distachyon. The YSL3 transporter plays a crucial role in copper distribution to reproductive organs and grains, impacting their development.
Addressing the looming global food security crisis requires the development of high-yielding crops. In agricultural soils, deficiency in the micronutrient copper significantly decreases grain yield in wheat (Triticum aestivum), a globally important crop. In cereals, grain yield is determined by inflorescence architecture, flower fertility, grain size, and weight. Whether copper is involved in these processes, and how it is delivered to the reproductive organs is not well understood. We show that copper deficiency alters not only the grain set but also flower development in both wheat and its recognized model, Brachypodium distachyon. We then show that the Brachypodium yellow stripe-like 3 (YSL3) transporter localizes to the phloem, transports copper in frog (Xenopus laevis) oocytes, and facilitates copper delivery to reproductive organs and grains. Failure to deliver copper, but not iron, zinc, or manganese to these structures in the ysl3 CRISPR-Cas9 mutant results in delayed flowering, altered inflorescence architecture, reduced floret fertility, grain size, weight, and protein accumulation. These defects are rescued by copper supplementation and are complemented by YSL3 cDNA. This knowledge will help to devise sustainable approaches for improving grain yield in regions where soil quality is a major obstacle for crop production. Copper distribution by a phloem-localized transporter is essential for the transition to flowering, inflorescence architecture, floret fertility, size, weight, and protein accumulation in seeds.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据