期刊
SCIENTIFIC REPORTS
卷 11, 期 1, 页码 -出版社
NATURE PORTFOLIO
DOI: 10.1038/s41598-021-97351-y
关键词
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资金
- Bio & Medical Technology Development Program of the National Research Foundation (NRF) - Korean government (MSIT) [2019R1F1A1059250, 2019M3E5D1A01069361, 2020R1I1A1A01069168]
- National Research Foundation of Korea (NRF) Basic Science Research Program, Ministry of Science, ICT & Future Planning [NRF-2017R1C1B5075941]
- Soonchunhyang University Research Fund
- National Research Foundation of Korea [2020R1I1A1A01069168, 2019R1F1A1059250, 2019M3E5D1A01069361] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Lineage tracing in mice has shown that LGR5 is an adult stem cell marker in various organs, and this study aimed to investigate its expression in human breast tissue. LGR5 expression was found in regenerative tissues, ductal carcinoma in situ, and ducts surrounded by invasive cancer cells, with a higher prevalence in triple-negative breast cancer cases. The study also revealed positive correlations with higher histologic grades and T stages, but negative associations with estrogen and progesterone receptor expression. Further research is needed to understand the functional and prognostic implications of LGR5 in triple-negative breast cancer.
Lineage tracing in mice indicates that LGR5 is an adult stem cell marker in multiple organs, such as the intestine, stomach, hair follicles, ovary, and mammary glands. Despite many studies exploring the presence of LGR5 cells in human tissues, little is known about its expression profile in either human mammary tissue or pathological lesions. In this study we aim to investigate LGR5 expression in normal, benign, and malignant lesions of the human breast using RNA in situ hybridization. LGR5 expression has not been observed in normal lactiferous ducts and terminal duct lobular units, whereas LGR5-positive cells have been specifically observed in the basal myoepithelium of ducts in the regenerative tissues, ductal carcinoma in situ, and in ducts surrounded by invasive cancer cells. These findings suggest LGR5 marks facultative stem cells that are involved in post injury regeneration instead of homeostatic stem cells. LGR5 positivity was found in 3% (9 of 278 cases) of invasive breast cancers (BC), and it showed positive associations with higher histologic grades (P = 0.001) and T stages (P < 0.001), while having negative correlations with estrogen receptor (P < 0.001) and progesterone receptor (P < 0.001) expression. Remarkably, all LGR5-positive BC, except one, belong to triple-negative BC (TNBC), representing 24% (9 of 38 cases) of all of them. LGR5 histoscores have no correlations with EGFR, CK5/6, Ki-67, or P53 expression. Additionally, no beta-catenin nuclear localization was observed in LGR5-positive BC, indicating that canonical Wnt pathway activation is less likely involved in LGR5 expression in BC. Our results demonstrate that LGR5 expression is induced in regenerative conditions in the myoepithelium of human mammary ducts and that its expression is only observed in TNBC subtype among all invasive BC. Further studies regarding the functional and prognostic impact of LGR5 in TNBC are warranted.
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