期刊
TECHNOLOGY IN CANCER RESEARCH & TREATMENT
卷 20, 期 -, 页码 -出版社
SAGE PUBLICATIONS INC
DOI: 10.1177/15330338211041193
关键词
nonsmall cell lung cancer; microRNA; overexpression; cell proliferation; apoptosis
类别
资金
- Zhejiang Medical Association Clinical Research Fund Project Emergency Critical Care Scientific Research Project [2019ZYC-A117]
The study revealed that microRNA-342-3p is downregulated in nonsmall cell lung cancer compared to normal cells and tissues. Overexpression of microRNA-342-3p suppressed cell proliferation and promoted apoptosis by repressing the expression of B-cell lymphoma-2, suggesting it as a potential target for nonsmall cell lung cancer treatment.
microRNA-342-3p plays an important role in tumor occurrence and development. However, the expression pattern and roles of microRNA-342-3p in nonsmall cell lung cancer remain poorly understood. In the current study, we explored the roles and underlying mechanisms of microRNA-342-3p in nonsmall cell lung cancer via gain- and loss-of-function analyses. We used quantitative reverse-transcription-polymerase chain reaction and western blotting assays to measure the expression levels of microRNA-342-3p in nonsmall-cell lung cancer and B-cell lymphoma-2. Furthermore, we used small interfering RNA and RNA mimics to analyze the functions and underlying mechanisms of microRNA-342-3p in nonsmall cell lung cancer cells. A luciferase reporter assay was performed to evaluate the direct binding site of the 5 '-untranslated region of B-cell lymphoma-2 targeted by microRNA-342-3p. We found that the expression of microRNA-342-3p was significantly lower in nonsmall cell lung cancer cells and tissues than in normal cells and tissues. The upregulation of microRNA-342-3p suppressed cell proliferation while promoting apoptosis in H1975, H460, and H226 cells. The overexpression of microRNA-342-3p in nonsmall cell lung cancer cells led to the downregulation of mRNA and protein levels in B-cell lymphoma-2 cells. Thus, B-cell lymphoma-2 was identified as a direct target of microRNA-342-3p. These findings indicate that microRNA-342-3p inhibits the growth of nonsmall cell lung cancer by repressing the expression of B-cell lymphoma-2, which suggests that microRNA-342-3p could be a potential target for the treatment of nonsmall cell lung cancer.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据