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Modified ELISA for antibody avidity evaluation: The need for standardization

期刊

BIOMEDICAL JOURNAL
卷 44, 期 4, 页码 433-438

出版社

ELSEVIER
DOI: 10.1016/j.bj.2020.10.009

关键词

Antibody avidity assay; Antibody; ELISA

资金

  1. Sao Paulo Research Foundation (FAPESP) [12/15568-0, 13/11147-2, 14/11172-0, 14/07182-0, 18/04202-0, 18/17945-1, 19/02042-9]
  2. National Council for Scientific and Technological Development [132743/2014-6, 131412/2019-1]
  3. Coordination of Improvement of Post-Graduate Level Personnel (CAPES)
  4. Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [12/15568-0, 19/02042-9] Funding Source: FAPESP

向作者/读者索取更多资源

Antibody avidity is a crucial parameter for evaluating immune response and distinguishing different infection statuses, with the most common method being a modified ELISA. The use of commercial kits or in-house methods to assess antibody avidity is increasing, but the lack of standardization between different assays can lead to variability in results.
Antibody avidity is an important parameter to evaluate immune response, being useful to evaluate vaccine responses and helping to distinguish acute and latent infection. The antibody avidity can be measured by different methods, yet the most common is a modified ELISA. The utilization of commercial kits or in-house methods to evaluate antibody avidity have been adopted more and more, although the lack of standardization between different assays may generate a lot of variation in the process, making it hard to compare the results generated.

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