期刊
PLANT CELL
卷 33, 期 9, 页码 3022-3041出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/plcell/koab168
关键词
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资金
- National Natural Science Foundation of China [31870258, 31922009]
- Yunnan Fundamental Research Projects [2018FA012, 2019FI006, 202001AV070009, 202001AT070118]
- CAS Light of West China Program
- Youth Innovation Promotion Association of the of Chinese Academy of Sciences [Y201973]
- Yunnan High Level Talents Special Support Plan [YNWR-QNBJ-2018-075]
- Candidates of the Youngand Middle Aged Academic Leaders of Yunnan Province [2017HB067]
Seed germination and postgerminative growth require precise coordination of multiple intrinsic and environmental signals. Core circadian clock proteins PRR5 and PRR7 physically associate with the crucial ABA signaling transcription factor ABI5, positively modulating ABA signaling during seed germination.
Seed germination and postgerminative growth require the precise coordination of multiple intrinsic and environmental signals. The phytohormone abscisic acid (ABA) suppresses these processes in Arabidopsis thaliana and the circadian clock contributes to the regulation of ABA signaling. However, the molecular mechanism underlying circadian clock-mediated ABA signaling remains largely unknown. Here, we found that the core circadian clock proteins PSEUDO-RESPONSE REGULATOR5 (PRR5) and PRR7 physically associate with ABSCISIC ACID-INSENSITIVE5 (ABI5), a crucial transcription factor of ABA signaling. PRR5 and PRR7 positively modulate ABA signaling redundantly during seed germination. Disrupting PRR5 and PRR7 simultaneously rendered germinating seeds hyposensitive to ABA, whereas the overexpression of PRR5 enhanced ABA signaling to inhibit seed germination. Consistent with this, the expression of several ABA-responsive genes is upregulated by PRR proteins. Genetic analysis demonstrated that PRR5 promotes ABA signaling mainly dependently on ABI5. Further mechanistic investigation revealed that PRR5 stimulates the transcriptional function of ABI5 without affecting its stability. Collectively, our results indicate that these PRR proteins function synergistically with ABI5 to activate ABA responses during seed germination, thus providing a mechanistic understanding of how ABA signaling and the circadian clock are directly integrated through a transcriptional complex involving ABI5 and central circadian clock components.
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