4.5 Article

Analysis of Dip2B Expression in Adult Mouse Tissues Using the LacZ Reporter Gene

期刊

CURRENT ISSUES IN MOLECULAR BIOLOGY
卷 43, 期 2, 页码 529-542

出版社

MDPI
DOI: 10.3390/cimb43020040

关键词

Dip2b; LacZ; nervous system; reproductive system; vascular system; respiratory system

资金

  1. National Natural Science Foundation of China [31301189, 81270953]
  2. Natural Science Foundation of Jilin Province [20160101344JC, 20200201127JC]

向作者/读者索取更多资源

Dip2B, a member of the Dip2 superfamily, plays a crucial role in axonal outgrowth during embryogenesis and is highly expressed in various somatic tissues in adults. Its broad and intense expression patterns in neuronal, reproductive, and vascular tissues, as well as in organs such as the kidneys, heart, liver, and lungs, suggest its involvement in numerous physiological functions.
Disconnected (disco)-interacting protein 2 homolog B (Dip2B) is a member of the Dip2 superfamily and plays an essential role in axonal outgrowth during embryogenesis. In adults, Dip2B is highly expressed in different brain regions, as shown by in situ analysis, and may have a role in axon guidance. However, the expression and biological role of Dip2B in other somatic tissues remain unknown. To better visualize Dip2B expression and to provide insight into the roles of Dip2B during postnatal development, we used a Dip2b(tm1a(wtsi)komp) knock-in mouse model, in which a LacZ-Neo fusion protein is expressed under Dip2b promoter and allowed Dip2B expression to be analyzed by X-gal staining. qPCR analyses showed that Dip2b mRNA was expressed in a variety of somatic tissues, including lung and kidney, in addition to brain. LacZ staining indicated that Dip2B is broadly expressed in neuronal, reproductive, and vascular tissues as well as in the kidneys, heart, liver, and lungs. Moreover, neurons and epithelial cells showed rich staining. The broad and intense patterns of Dip2B expression in adult mice provide evidence of the distribution of Dip2B in multiple locations and, thereby, its implication in numerous physiological roles.

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