4.8 Article

Structure of cell-cell adhesion mediated by the Down syndrome cell adhesion molecule

出版社

NATL ACAD SCIENCES
DOI: 10.1073/pnas.2022442118|1of12

关键词

DSCAM; cell adhesion; electron tomography; IgSF; cell-cell interaction

资金

  1. National Natural Science Foundation of China [31470735, 31670747, 91957102]

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DSCAM, a member of the immunoglobulin superfamily, plays crucial roles in neural development by mediating cell adhesion and self-avoidance of neurites. Through electron microscopy and additional methods, it has been found that DSCAM forms a regular pattern at cell adhesion interfaces, with Ig-like domains contributing to interactions and FnIII domains crucial for pattern formation and cell membrane interaction. On the other hand, no obvious assembly pattern is observed with other DSCAMs, indicating different structural roles in cell adhesion and neural network formation.
The Down syndrome cell adhesion molecule (DSCAM) belongs to the immunoglobulin superfamily (IgSF) and plays important roles in neural development. It has a large ectodomain, including 10 Ig-like domains and 6 fibronectin III (FnIII) domains. Previous data have shown that DSCAM can mediate cell adhesion by forming homophilic dimers between cells and contributes to self-avoidance of neurites or neuronal tiling, which is important for neural network formation. However, the organization and assembly of DSCAM at cell adhesion interfaces has not been fully understood. Here we combine electron microscopy and other biophysical methods to characterize the structure of the DSCAM-mediated cell adhesion and generate threedimensional views of the adhesion interfaces of DSCAM by electron tomography. The results show that mouse DSCAM forms a regular pattern at the adhesion interfaces. The Ig-like domains contribute to both trans homophilic interactions and cis assembly of the pattern, and the FnIII domains are crucial for the cis pattern formation as well as the interaction with the cell membrane. By contrast, no obvious assembly pattern is observed at the adhesion interfaces mediated by mouse DSCAML1 or Drosophila DSCAMs, suggesting the different structural roles and mechanisms of DSCAMs in mediating cell adhesion and neural network formation.

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