期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 85, 期 9, 页码 1932-1936出版社
OXFORD UNIV PRESS
DOI: 10.1093/bbb/zbab129
关键词
2-furanmethanethiol; LC-ESI-MS; volatile thiol compounds; monobromobimane; coffee aroma
类别
资金
- JSPS KAKENHI [JP19H05679, JP19H05687, JP19K22327, JP19K15727]
- Biooriented Technology Research Advancement Institution, Moonshot Agriculture, Forestry and Fisheries Research and Development Program [JPJ009237]
- JST Program on Open Innovation Platform with Enterprises, Research Institute and Academia [JPMJOP1832]
- euglena Co., Ltd.
The study evaluated the pretreatment of volatile thiols by S-bimanyl derivatization using monobromobimane to enable detection as nonvolatile derivatives. A convenient and efficient method was successfully developed for the quantitative analysis of 2-furanmethanethiol (a volatile thiol odorant of coffee aroma) in coffee beans.
Generally, volatile thiols are hard to be measured with electrospray-ionization-type LC-MS due to the volatility. Therefore, we here evaluated the pretreatment of their S-bimanyl derivatization by monobromobimane to enable the detection as nonvolatile derivative. Consequently, we successfully developed the convenient and efficient method through the quantitative analysis of 2-furanmethanethiol (volatile thiol odorant of coffee aroma) in coffee bean.
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