期刊
GENERAL PHYSIOLOGY AND BIOPHYSICS
卷 40, 期 5, 页码 351-363出版社
AEPRESS SRO
DOI: 10.4149/gpb_2021021
关键词
Endometrial receptivity; miR-494-3p; PI3K/AKT/mTOR pathway; Leukemia inhibitory factor
资金
- Xinglin Scholar Research Premotion Project of Chengdu University of TCM [2019yky08]
The study demonstrates that miR-494-3p regulates endometrial receptivity in mice through multiple protein expressions and signaling pathways, suggesting its significant biological function and potential clinical relevance.
Successful implantation requires endometrial receptivity. To investigate the mechanisms of miR-494-3p on endometrial receptivity GnRHa's superovulation scheme was designed to reduce endometrial receptivity, and the pregnant mice were injected with miR-494-3p antagomir. The regulatory role of miR-494-3p was identified by RT-qPCR, uterine blastocyst count, scanning electron microscopy, hematoxylin-eosin (HE) staining, and Western blot. Results indicated that miR-494-3p antagomir increased uterine blastocysts numbers, promoted the pinocytosis expressions, and increased endometrial thickness. Besides, miR-494-3p antagomir significantly increased leukemia inhibitory factor (LIF), Ang-2 and VEGF protein expressions, and up-regulated p-AKT/AKT and p-mTOR/mTOR protein ratios in endometrium. Luciferase assay confirmed that LIF was a potential target of miR-494-3p. Subsequently, human endometrial epithelial cells (hEECs) were transfected with miR-494-3p inhibitor and PI3K inhibitor (LY294002). The role of miR-494-3p was identified by RT-qPCR, CCK-8 assay, transwell assay and flow cytometry. Results indicated that miR-494-3p inhibitor significantly increased proliferation and invasion, and significantly inhibited apoptosis in hEECs, while LY294002 reversed its biological function. Overall, these results suggested that miR-494-3p is the key regulator of endometrial receptivity in mice, regulating this complex process through the PI3K/AKT/TOR pathway. Understanding the role of miR-494-3p in endometrial receptivity is of great significance for exploring new targets for the diagnosis and treatment of early pregnancy failure, and improving the success rates of artificial reproduction.
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