期刊
CHINESE JOURNAL OF NATURAL MEDICINES
卷 19, 期 10, 页码 758-771出版社
CHINESE JOURNAL NATURAL MEDICINES
DOI: 10.1016/S1875-5364(21)60082-1
关键词
Berberis amurensis; Berberidaceae; Pseudo germ-free rat; Intestinal flora; UPLC; Q-TOF-MS; MS
资金
- National Key R&D Program of China [2018YFC1708203]
- Non-profit Central Research Institute Fund of Chinese Academy of Medical Sciences [2018PT35031]
- Drug Innovation Major Project [2018ZX09711001-002-002]
- Major Science and Technology Project of Inner Mongolia Autonomous Region [2019ZD004]
The study showed that the metabolism pathways of Berberis amurensis extracts (BAEs) differed between normal rats and pseudo germ-free rats, indicating that intestinal bacteria have an influence on the metabolism of BAEs.
Berberis amurensis (Berberidaceae) is a traditional Chinese medicine, which is often used to treat hypertension, inflammation, dysentery and enteritis. It contains alkaloids, mainly including berberine, berbamine, magnoflorine, jatrorrhizine and palmatine. Berberis amurensis extracts (BAEs) is often orally taken. Oral herbs might be metabolized by intestinal bacteria in the small intestine. However, the interaction between the herb and the gut microbiota is still unknown. In the current study, UPLC/Q-TOFMS/MS combined with Metabolitepilot and Peakview software was used to identify the metabolites of BAEs in anti-biotic cocktail induced pseudo germ-free rats and normal rats. As a result, a total of 46 metabolites in normal rats were detected and its main metabolic pathways include demethylation, dehydrogenation, methylation, hydroxylation, sulfation and glucuronidation. Only 29 metabolites existed in pseudo germ-free rats. Dehydrogenated metabolites (M29, M30, M34 and M36), methylated metabolites (M33, M41 and M46) and other metabolites were not detected in pseudo germ-free rats. The result implied that the intestinal bacteria have an influence on the metabolism of BAEs. Furthermore, this investigation might contribute to the understanding of the metabolism of BAEs, and further promote its clinical application.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据