期刊
SIGNAL TRANSDUCTION AND TARGETED THERAPY
卷 6, 期 1, 页码 -出版社
SPRINGERNATURE
DOI: 10.1038/s41392-021-00800-3
关键词
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资金
- National Key R&D Program of China [2020YFA0707600, 2020YFA0707800]
- National Natural Science Foundation of China [81930063, 31870893, 81971948]
- National Major Sciences & Technology Project for Control and Prevention of Major Infectious Diseases in China [2018ZX10301401]
- Beijing Municipal Science & Technology Commission [Z181100001318009]
- Chinese Academy of Medical Sciences (CAMS) Innovation Fund for Medical Sciences [2016-I2M-1-014, 2016-I2M-1-005]
- Beijing Advanced Innovation Center for Genomics (ICG) at Peking University
- Peking-Tsinghua Center for Life Sciences
SARS-CoV-2 infection activates the host innate immune response through cytoplasmic DNA sensing pathway, involving cGAS and STING. Research shows that chromatin DNA can be transferred from the nucleus to the cytoplasm upon infection, triggering an immune alarm response. The study also highlights the essential roles of cGAS and STING in combating SARS-CoV-2, with potential therapeutic implications.
The global coronavirus disease 2019 (COVID-19) pandemic is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a positive-sense RNA virus. How the host immune system senses and responds to SARS-CoV-2 infection remain largely unresolved. Here, we report that SARS-CoV-2 infection activates the innate immune response through the cytosolic DNA sensing cGAS-STING pathway. SARS-CoV-2 infection induces the cellular level of 2 ' 3 '-cGAMP associated with STING activation. cGAS recognizes chromatin DNA shuttled from the nucleus as a result of cell-to-cell fusion upon SARS-CoV-2 infection. We further demonstrate that the expression of spike protein from SARS-CoV-2 and ACE2 from host cells is sufficient to trigger cytoplasmic chromatin upon cell fusion. Furthermore, cytoplasmic chromatin-cGAS-STING pathway, but not MAVS-mediated viral RNA sensing pathway, contributes to interferon and pro-inflammatory gene expression upon cell fusion. Finally, we show that cGAS is required for host antiviral responses against SARS-CoV-2, and a STING-activating compound potently inhibits viral replication. Together, our study reported a previously unappreciated mechanism by which the host innate immune system responds to SARS-CoV-2 infection, mediated by cytoplasmic chromatin from the infected cells. Targeting the cytoplasmic chromatin-cGAS-STING pathway may offer novel therapeutic opportunities in treating COVID-19. In addition, these findings extend our knowledge in host defense against viral infection by showing that host cells' self-nucleic acids can be employed as a danger signal to alarm the immune system.
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